A Radioimmunoassay for 3,5,3′-Triiodothyronine in Chicken Serum
نویسندگان
چکیده
منابع مشابه
Radioimmunoassay of triiodothyronine in unextracted human serum.
Serum triiodothyronine (T-3) concentrations have been estimated by radioimmunoassay using unextracted serum. The serum T-3 concentrations have been shown to be similar in two separate European populations (0.76-1.67 ng/ml). Raised T-3 values have been observed in all subjects with hyperthyroidism. Low values are seen in hypothyroidism although there is some overlap with the normal range. There ...
متن کاملA two-step radioimmunoassay for free triiodothyronine in serum.
Using a high-affinity solid-phase-bound antibody (Ka = 1.2 X 10(11) L/mol), we have standardized a two-step radioimmunoassay for free triiodothyronine (FT3) in serum, based on immunoextraction. The method was validated by comparison with an equilibrium-dialysis procedure (r = 0.96) involving RIA of T3 in the dialysate standardized with the same antibody and by a commercial (Liso-Phase, Internat...
متن کاملRadioimmunoassay for measurement of triiodothyronine in human serum.
A convenient, specific, precise, and reproducible radioimmunoassay system for measurement of triiodothyronine (T(3)) in human serum has been developed. The procedure compares the ability of standards and unknowns to compete with radioactive T(3) for binding sites on a T(3)-binding antiserum produced in rabbits by immunization with human thyroglobulin. The assay is set up in the presence of 250 ...
متن کاملTriiodothyronine radioimmunoassay.
Highly specific antisera to triiodothyronine (T(3)) were prepared by immunization of rabbits with T(3)-bovine serum albumin conjugates. Antisera with T(3) binding capacity of up to 600 ng/ml were obtained. The ability of various thyronine derivatives to inhibit the binding of T(3-) (125)I to anti-T(3) serum was found to vary considerably. l-T(3), d-T(3) and several triiodoanalogues were potent ...
متن کاملRapid radioimmunoassay of triiodothyronine.
The method used in this report is based on the immunological production of a specific antisera to tnlodothyronine (T3) in rabbits. Conducted at 4 #{176}C, the radioimmunoassay requires three to five days to complete. Hoping to speed this assay, we investigated the influence of time, temperature, and pH. Incubation at 37 #{176}C and pH 8.0 for 30 mm did not deleteriously affect either precision,...
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ژورنال
عنوان ژورنال: Poultry Science
سال: 1978
ISSN: 0032-5791
DOI: 10.3382/ps.0571740